Development and validation of RP-HPLC method for quantification of trace levels of topical corticosteroids in ambiphilic cream

Abstract Corticosteroids are anti-inflammatory and immunosuppressant drugs. Topical corticosteroids formulations (ointments, creams, gels) used in the treatment of different types dermatitis urticaria. Considering their therapeutic whitening effects, they frequently for counterfeiting cosmetic products. can cause local systemic side effects. HPLC method is often chosen analysis, because it selective, sensitive, precise, simple fast. The aim this study was optimization validation RP-HPLC with UV detection determination trace levels ambiphilic creams. This qualitative quantitative analysis evaluated corticosteroids. Mometasone furoate, hydrocortisone acetate, fluocinonide, fluocinolone acetonide, betamethasone, betamethasone dipropionate triamcinolone acetonide were evaluated. Separation performed on Inertsil® ODS-3V 250 × 4.6 mm, 5 μm chromatographic column. Mobile phase mixture acetonitrile water 50:50 (v/v) gradient elution flow rate 1 mL min −1 . Column temperature held 40 °C at 240 nm. Selectivity, linearity, accuracy, precision limit quantification (LOQ) Method selective cream base peaks not overlapping. Linearity confirmed since correlation coefficient all compounds. Accuracy acetate dipropionate. Determined Recovery values range 70–130%. Both RSD (21.46% 9.59%) lower than 30%. highly sensitive LOQ concentrations ng range. All parameters accordance regulatory requirements. Validated be selected